Petukhov V G, Osin N S
Tsitologiia. 1983 Mar;25(3):345-8.
The technique of registering the afterglow of cells is described. Data are presented on spectral-luminescent characteristics of HeLa cells at room temperature under conditions of deep hypoxia, the residual pressure of oxygen in the medium being under 1 mmHg. The afterglow in the visible part of spectrum is shown to be due to protein tryptophanils with phosphorescence peaks at 415, 445, and 470 nm; metalloporphyrins with delayed fluorescence peaks at 595 and 640 nm, as well as by other cellular components with peaks at about 500 and 540 nm. The latter components are supposed to be NAD, NADH and flavins. The passage of air through the suspension of cells eliminating hypoxia results in practically a complete extinction of the afterglow of cells.
描述了记录细胞余辉的技术。给出了在深度缺氧条件下（培养基中氧气残余压力低于1 mmHg），HeLa细胞在室温下的光谱发光特性数据。光谱可见部分的余辉被证明是由于具有在415、445和470 nm处磷光峰的蛋白质色氨酸；具有在595和640 nm处延迟荧光峰的金属卟啉，以及具有在约500和540 nm处峰的其他细胞成分。后一种成分被认为是NAD、NADH和黄素。空气通过细胞悬液消除缺氧会导致细胞余辉几乎完全熄灭。
