Lowenhaupt K, Lingrel J B
Cell. 1978 Jun;14(2):337-44. doi: 10.1016/0092-8674(78)90119-8.
The stability of globin mRNA in murine erythroleukemia cells (Friend cells) before and during DMSO-induced differentiation was investigated. Cells were exposed to 3H-uridine for 2 hr and then transferred to medium without the radioactive precursor. The loss of radioactivity in total RNA, poly(A)-containing RNA and globin mRNA was followed. The globin mRNA was isolated using a highly specific globin cDNA column. In uninduced cells and cells early in differentiation, the globin mRNA decays with a half-life of less than 50 hr. After 4 days of induction, the globin mRNA decays with a half-life of 17 hr, demonstrating a change in stability during the induction process. Although the stability of globin mRNA changes during induction, this is not true for total poly(A)-containing RNA. At all times of induction, the poly(A)-containing RNA decays as two populations, one with a half-life of 6 hr and the other with a half-life of 36 hr. The half-life of the rRNA also remains unchanged during differentiation.
研究了二甲基亚砜（DMSO）诱导分化之前及过程中，小鼠红白血病细胞（Friend细胞）中珠蛋白mRNA的稳定性。将细胞暴露于3H-尿苷中2小时，然后转移至不含放射性前体的培养基中。跟踪总RNA、含聚腺苷酸（poly(A)）的RNA和珠蛋白mRNA中放射性的损失情况。使用高度特异性的珠蛋白cDNA柱分离珠蛋白mRNA。在未诱导的细胞和分化早期的细胞中，珠蛋白mRNA的半衰期小于50小时。诱导4天后，珠蛋白mRNA的半衰期为17小时，表明在诱导过程中稳定性发生了变化。尽管诱导过程中珠蛋白mRNA的稳定性发生了变化，但对于总的含poly(A)的RNA来说并非如此。在诱导的所有阶段，含poly(A)的RNA以两个群体的形式衰变，一个半衰期为6小时，另一个半衰期为36小时。在分化过程中，核糖体RNA（rRNA）的半衰期也保持不变。
