终末分化的小鼠红白血病细胞中珠蛋白mRNA的稳定性

Stability of globin mRNA in terminally differentiating murine erythroleukemia cells.

作者信息

Volloch V, Housman D

出版信息

Cell. 1981 Feb;23(2):509-14. doi: 10.1016/0092-8674(81)90146-x.

Abstract

The stability of globin mRNA is terminally differentiating MEL cells has been reevaluated. Previously, it had been reported that globin mRNA has a half-life of approximately 17 hr in terminally differentiating MEL cells. We show that the previous measurements of this parameter were confounded by physical instability of differentiating MEL cells. By using culture conditions that physically stabilize end-stage cells we show that the stability of globin mRNA in terminally differentiating MEL cells is equal to the value observed for ribosomal RNA, a half-life greater than 60 hr.

摘要

已对珠蛋白mRNA在终末分化的MEL细胞中的稳定性进行了重新评估。此前有报道称，在终末分化的MEL细胞中，珠蛋白mRNA的半衰期约为17小时。我们发现，之前对该参数的测量受到了分化中的MEL细胞物理不稳定性的干扰。通过使用能使终末阶段细胞在物理上保持稳定的培养条件，我们发现，在终末分化的MEL细胞中，珠蛋白mRNA的稳定性与核糖体RNA的稳定性相当，半衰期超过60小时。