Wakayama Y, Matsuzaki H, Nakai Y
Dev Neurosci. 1983;6(3):152-60. doi: 10.1159/000112342.
The localization of acid phosphatase activity has been investigated ultrastructurally in regenerating human fetal skeletal muscle cells. The heavy reaction product was observed in the cisternae of Golgi apparatus and lysosomal vesicles, whereas the slight reaction product was also occasionally found in the nuclear envelope, endoplasmic reticulum and sarcoplasmic reticulum of the regenerating myocytes. The enzymatic basis for these deposits was confirmed by their absence in the incubation of the specimen in substrate-free medium and the enzyme inactivation of the specimen by exposure to NaF or heat.
已利用超微结构研究了人胎儿骨骼肌细胞再生过程中酸性磷酸酶活性的定位。在高尔基体的潴泡和溶酶体小泡中观察到强烈的反应产物，而在再生肌细胞的核膜、内质网和肌浆网中偶尔也发现轻微的反应产物。通过在无底物培养基中孵育标本时这些沉积物的缺失以及通过暴露于氟化钠或加热使标本酶失活，证实了这些沉积物的酶学基础。
