Method for an increase of the number of anaphases in Ehrlich ascites tumour cells in mouse.

A method causing an increase of the number of anaphases of animal cells in vivo, suitable for mutagenicity testing, is presented. The Ehrlich ascites cells transplanted in mice were used as test cells. The increase of the number of anaphases (8-23/1000) was obtained by: 1. transplantation of the ascites tumour on 5th or more days of tumour growth from the 1st to the 2nd mouse; 2. by incubation of ascites tumour cells in the peritoneal cavity of the 2nd mouse; by incubation of ascites tumour cells in the peritoneal cavity of the 2nd mouse for 4 h. The percentage of abnormal anaphases of Ehrlich ascites tumour cells not treated with mutagen was 3.19 +/- 3.1. The increase of the number of anaphases was the result of an increase of mitotic index after transplantation of ascites tumour cells to a new host at the time of the decline of cell proliferation.