Zuyderhoudt F M, Hengeveld P, van Gool J, Jörning G G
Clin Chim Acta. 1978 Jun 15;86(3):313-21. doi: 10.1016/0009-8981(78)90387-x.
Methods are described for measurement of total tissue iron, ferritin iron, haem iron and ferritin protein in approx. 15 mg of tissue obtained by liver biopsy. The validity of these methods is examined by comparison with the values observed in larger samples of the same post-mortem derived liver tissue. Correlation coefficients vary between 0.80 and 0.99 (n = 11--16). It appears that in post-mortem liver tissue the haem iron concentration is higher than in biopsy specimens from patients. Analysis of liver biopsy specimens from patients with hepatitis showed a large variation in the mean iron content of the liver ferritin molecules. Also, the non-ferritin depot iron concentration and ferritin protein concentration is quite variable. It is suggested that in cases of advanced ferritin catabolism during hepatitis the mean percentage of iron in ferritin molecules often increases while at the same time the non-ferritin depot iron fraction decreases, probably because of iron release from the liver.
本文描述了测量约15mg肝活检组织中总组织铁、铁蛋白铁、血红素铁和铁蛋白的方法。通过与取自相同尸检肝脏组织的更大样本中观察到的值进行比较，检验了这些方法的有效性。相关系数在0.80至0.99之间（n = 11 - 16）。尸检肝脏组织中的血红素铁浓度似乎高于患者的活检标本。对肝炎患者肝活检标本的分析显示，肝脏铁蛋白分子的平均铁含量存在很大差异。此外，非铁蛋白储存铁浓度和铁蛋白浓度也变化很大。有人提出，在肝炎期间铁蛋白分解代谢晚期，铁蛋白分子中铁的平均百分比常常增加，而与此同时非铁蛋白储存铁部分减少，这可能是由于肝脏中铁的释放。
