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人肝穿刺活检标本中铁蛋白的分离与测定

Separation and assay of iron proteins in needle biopsy specimens of human liver.

作者信息

Selden C, Peters T J

出版信息

Clin Chim Acta. 1979 Oct 15;98(1-2):47-52. doi: 10.1016/0009-8981(79)90164-5.

DOI:10.1016/0009-8981(79)90164-5
PMID:498529
Abstract

A micro method has been developed for the separation of the principal classes of iron proteins in needle biopsy specimens of human liver. The iron content of the fractions was determined by automated flameless atomic absorption spectrophotometry in a one step procedure. The levels of total iron, transferrin-, ferritin-, haemprotein- and haemosiderin-iron are reported for control tissue.

摘要

已开发出一种微量方法,用于分离人肝穿刺活检标本中主要种类的铁蛋白。通过一步法自动无火焰原子吸收分光光度法测定各组分的铁含量。报告了对照组织中总铁、转铁蛋白铁、铁蛋白铁、血红蛋白铁和含铁血黄素铁的水平。

相似文献

1
Separation and assay of iron proteins in needle biopsy specimens of human liver.人肝穿刺活检标本中铁蛋白的分离与测定
Clin Chim Acta. 1979 Oct 15;98(1-2):47-52. doi: 10.1016/0009-8981(79)90164-5.
2
Studies on the concentration and intracellular localization of iron proteins in liver biopsy specimens from patients with iron overload with special reference to their role in lysosomal disruption.对铁过载患者肝活检标本中铁蛋白的浓度和细胞内定位的研究,特别关注其在溶酶体破坏中的作用。
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[Total heminic and non-heminic liver iron in normal and castrated rats. II].
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Quantitative determination of non-haem iron and ferritin iron in bone marrow using flameless atomic absorption spectrophotometry. A comparative study on the cytological and chemical determination of the bone marrow iron content.使用无火焰原子吸收分光光度法对骨髓中的非血红素铁和铁蛋白铁进行定量测定。骨髓铁含量的细胞学和化学测定的比较研究。
Eur J Clin Chem Clin Biochem. 1991 Jun;29(6):381-4. doi: 10.1515/cclm.1991.29.6.381.

引用本文的文献

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The value of magnetic resonance imaging in evaluation of myocardial and liver iron overload in a thalassaemia endemic population: a report from Northeastern Thailand.磁共振成像在泰国东北部地中海贫血流行人群中心肌和肝脏铁过载评估中的价值:一份报告
Pol J Radiol. 2019 May 30;84:e262-e268. doi: 10.5114/pjr.2019.86094. eCollection 2019.
2
Iron overload cardiomyopathy: better understanding of an increasing disorder.铁过载性心肌病:对一种日益增多的疾病的更好理解。
J Am Coll Cardiol. 2010 Sep 21;56(13):1001-12. doi: 10.1016/j.jacc.2010.03.083.
3
Iron overload cardiomyopathies: new insights into an old disease.
铁过载心肌病:对一种古老疾病的新见解。
Cardiovasc Drugs Ther. 1994 Feb;8(1):101-10. doi: 10.1007/BF00877096.
4
Biochemical studies on the isolation and characterization of human spleen haemosiderin.关于人脾脏含铁血黄素的分离与特性的生化研究。
Biochem J. 1984 Oct 1;223(1):31-8. doi: 10.1042/bj2230031.
5
Characterization of isolated Fe-loaded rat hepatocytes prepared by collagenase perfusion.通过胶原酶灌注制备的分离的铁负载大鼠肝细胞的特性分析。
In Vitro. 1982 Nov;18(11):945-51. doi: 10.1007/BF02796351.
6
Formation of hydroxyl radicals in the presence of ferritin and haemosiderin. Is haemosiderin formation a biological protective mechanism?铁蛋白和含铁血黄素存在时羟基自由基的形成。含铁血黄素的形成是一种生物保护机制吗?
Biochem J. 1986 Mar 15;234(3):727-31. doi: 10.1042/bj2340727.
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Iron release from haemosiderin and production of iron-catalysed hydroxyl radicals in vitro.体外铁蛋白中铁的释放及铁催化羟基自由基的产生。
Biochem J. 1988 Mar 1;250(2):589-95. doi: 10.1042/bj2500589.
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Iron release from haemosiderin and ferritin by therapeutic and physiological chelators.治疗性螯合剂和生理性螯合剂从含铁血黄素和铁蛋白中释放铁。
Biochem J. 1989 Jun 15;260(3):903-7. doi: 10.1042/bj2600903.