Bush R K, Voss M J, Bashirian S
J Allergy Clin Immunol. 1983 Feb;71(2):239-44. doi: 10.1016/0091-6749(83)90105-7.
Allergens involved in sensitivity to Alternaria have not been well defined. We used crossed radioimmunoelectrophoresis (CRIE) to study antigens in a crude A. alternata extract, which were capable of binding IgE in human serum. Sera from 35 patients sensitive to Alternaria, 10 not sensitive to Alternaria, and five normal controls were examined. CRIE with hyperimmune rabbit sera demonstrated 22 antigens in the Alternaria extract. After exposure of CRIE gels sequentially with patient serum and 125I-labeled anti-human IgE, autoradiography demonstrated that three of the antigens bound IgE in sera of Alternaria-sensitive subjects. Our results suggest that multiple allergens are involved in A. alternata sensitivity.
与链格孢菌敏感性相关的变应原尚未得到明确界定。我们采用交叉放射免疫电泳（CRIE）来研究链格孢菌粗提物中的抗原，这些抗原能够与人血清中的IgE结合。检测了35名对链格孢菌敏感的患者、10名对链格孢菌不敏感的患者以及5名正常对照者的血清。用超免疫兔血清进行的CRIE显示链格孢菌提取物中有22种抗原。在CRIE凝胶依次与患者血清和125I标记的抗人IgE接触后，放射自显影显示其中三种抗原能与链格孢菌敏感受试者血清中的IgE结合。我们的结果表明，多种变应原与链格孢菌敏感性有关。
