Touzeau G, Kato A C
Neurology. 1983 Mar;33(3):317-22. doi: 10.1212/wnl.33.3.317.
Dissociated monolayer cultures of chick ciliary ganglion neurons have been used to study the effects of control and ALS sera. The cultured neurons survive and extend neurites for a minimum of 2 weeks in a standard tissue culture medium that contains 10% heat-inactivated human serum. Three parameters of the neurons have been examined when cultured in control and ALS sera for 8 to 12 days: (1) neuronal survival, (2) activity of the enzyme choline acetyltransferase, and (3) synthesis of 3H-acetylcholine using 3H-choline as precursor. ALS sera cause a small decrease in these three parameters, but this difference is not significant.
