Hinrichs U, Karzel K
Arzneimittelforschung. 1983;33(1):143-9.
Cultures of murine fetal bone explants were used as experimental model for the investigation of drug effects on mesenchymal metabolic processes, particularly the collagen metabolism. As parameters for growth or metabolic processes, resp., the increase in size, the DNA-, uronic acid- and hydroxyproline content of the explants were determined under various modifications of the culture conditions. Addition of ascorbic acid to the culture medium caused in concentrations from 2.5 micrograms/ml an increase of the hydroxyproline content and of the size and in concentrations from 20 or 40 micrograms/ml, resp., also an increase of the DNA- and uronic acid content of the explants. Proline exerted in concentrations up to 100 micrograms/ml no significant influences on the determined parameters and showed in concentrations from 150 micrograms/ml inhibitory effects. Substitution of the horse serum of the culture medium by chick embryonic extract resulted in a decrease of growth and metabolic activity of the explants. Under the influence of an oxygen content of 10% in the gas phase of the culture system all determined parameters with exception of the DNA-content were significantly increased as compared with 20% oxygen content, whereas concentrations of 30% oxygen or more led to reduction of growth and uronic acid content of the explants.
将小鼠胎儿骨外植体培养物用作实验模型，以研究药物对间充质代谢过程，特别是胶原代谢的影响。作为生长或代谢过程的参数，分别在不同的培养条件下测定外植体的大小增加、DNA、糖醛酸和羟脯氨酸含量。向培养基中添加抗坏血酸，浓度为2.5微克/毫升时会导致羟脯氨酸含量和大小增加，浓度为20或40微克/毫升时，分别还会导致外植体的DNA和糖醛酸含量增加。脯氨酸浓度高达100微克/毫升时对所测定的参数没有显著影响，而浓度为150微克/毫升时则表现出抑制作用。用鸡胚提取物替代培养基中的马血清会导致外植体的生长和代谢活性降低。与20%氧气含量相比，在培养系统气相中氧气含量为10%的影响下，除DNA含量外，所有测定参数均显著增加，而30%或更高的氧气浓度会导致外植体的生长和糖醛酸含量降低。
