Ramp W K, Arnold R R, Russell J E, Yancey J M
J Periodontol. 1987 May;58(5):340-4. doi: 10.1902/jop.1987.58.5.340.
Effects of H2O2 on bone were evaluated in an organ culture system. Tibiae from chick embryos were incubated for up to 3 days in culture medium containing 0.07 to 20 mM H2O2. Glucose metabolism was monitored by measuring lactate production and oxygen consumption, and collagen synthesis was determined by hydroxylation of proline. In addition to markedly inhibiting these parameters, H2O2 also decreased bone weight and alkaline phosphatase activity. Multiple exposures to H2O2 were somewhat more effective than a single exposure. Since H2O2 inhibits bone at low concentrations in vitro, the results suggest that the potential for harmful effects of H2O2 in the oral cavity should be investigated.
在器官培养系统中评估了过氧化氢（H2O2）对骨骼的影响。将鸡胚的胫骨在含有0.07至20 mM H2O2的培养基中培养长达3天。通过测量乳酸生成和氧气消耗来监测葡萄糖代谢，并通过脯氨酸的羟基化来测定胶原蛋白合成。除了显著抑制这些参数外，H2O2还降低了骨骼重量和碱性磷酸酶活性。多次暴露于H2O2比单次暴露更有效。由于H2O2在体外低浓度时就能抑制骨骼，结果表明应研究H2O2在口腔中产生有害影响的可能性。
