Raaphorst G P, Azzam E I
Cancer Res. 1983 Jun;43(6):2618-21.
Three normal human fibroblast strains, two human ataxia telangiectasia heterozygote cell strains, and two human ataxia telangiectasia homozygote cell strains were studied for their thermal responses between 41.0 and 46.0 degrees. The heat sensitivities of all cell strains were comparable, and all cell strains were relatively heat resistant compared to Chinese hamster cells. Both normal and ataxia telangiectasia human cells developed thermal tolerance during heating at temperatures less than or equal to 43 degrees and during incubation at 37 degrees after acute heating at 45.0 degrees. For survival measured down to the 5 to 10% level, heat survival curves for all seven human cell strains lacked shoulders, indicating the inability of such cells to accumulate sublethal heat damage. Analysis of the cell survival curve data by the method of Arrhenius showed that the thermal inactivation energies for human cells were 127 and 230 kcal/mol above and below the break at 43.5 degrees, respectively, and are about the same as for Chinese hamster cells and other animal cells, implying similar mechanisms of heat inactivation.
研究了三株正常人成纤维细胞系、两株人类共济失调毛细血管扩张症杂合子细胞系和两株人类共济失调毛细血管扩张症纯合子细胞系在41.0至46.0摄氏度之间的热反应。所有细胞系的热敏感性相当，与中国仓鼠细胞相比，所有细胞系都具有相对耐热性。正常人和共济失调毛细血管扩张症患者的人类细胞在温度低于或等于43摄氏度的加热过程中以及在45.0摄氏度急性加热后于37摄氏度孵育期间都产生了热耐受性。对于低至5%至10%水平的存活率测量，所有七株人类细胞系的热存活曲线都没有肩部，表明此类细胞无法积累亚致死性热损伤。通过阿伦尼乌斯方法对细胞存活曲线数据进行分析表明，人类细胞在43.5摄氏度断点以上和以下的热失活能分别为127和230千卡/摩尔，与中国仓鼠细胞和其他动物细胞的热失活能大致相同，这意味着热失活机制相似。
