Tam P P, Ng T B, Woo N Y
Cell Tissue Res. 1983;231(3):579-92. doi: 10.1007/BF00218116.
The pituitary gland of the red grouper, Epinephelus akaara, was studied by histochemical techniques, and the prolactin cells, corticotrops, somatotrops, gonadotrops, thyrotrops, pars intermedia cells and neurohypophyseal cells, were identified. Oestradiol-17 beta treatment caused PAS-positive cells in the proximal pars distalis, presumably a mixture of gonadotrops and thyrotrops, to undergo hypertrophy, vacuolation and degranulation of cytoplasmic glycoprotein granules. Disappearance of cytoplasmic granules was also evident in the PAS-positive pars intermedia cells. Oestrogen-treated fish also showed an increase in the hepatosomatic index, and hepatocytes enlarged in size, their nuclear diameter increased and large vacuoles were formed in the cytoplasm. These changes in the liver were paralleled by a secretion of vitellogenin into the serum and an increased production of mucus by the thickened skin epithelium. Testosterone injections did not affect such changes, neither in the pituitary nor liver cells, but a proliferation of skin epithelial cells was noted. Neither oestradiol-17 beta nor testosterone stimulated ovarian incorporation of vitellogenin, but treatment with high doses (5 mg/kg) of oestradiol-17 beta or testosterone brought about a slight increase in the gonadosomatic index and atresia of some of the primary oocytes. The oogonial population size decreased in response to treatment with high doses of oestradiol-17 beta.
采用组织化学技术对赤点石斑鱼（Epinephelus akaara）的脑垂体进行了研究，并鉴定出了催乳素细胞、促肾上腺皮质激素细胞、生长激素细胞、促性腺激素细胞、促甲状腺激素细胞、垂体中间部细胞和神经垂体细胞。17β-雌二醇处理使远侧部近端的PAS阳性细胞（可能是促性腺激素细胞和促甲状腺激素细胞的混合物）发生肥大、空泡化以及细胞质糖蛋白颗粒脱粒。垂体中间部的PAS阳性细胞中也明显出现细胞质颗粒消失的情况。经雌激素处理的鱼还表现出肝体指数增加，肝细胞体积增大，细胞核直径增大，细胞质中形成大空泡。肝脏的这些变化伴随着血清中卵黄蛋白原的分泌以及增厚的皮肤上皮黏液分泌增加。注射睾酮对垂体和肝细胞的这些变化均无影响，但观察到皮肤上皮细胞增殖。17β-雌二醇和睾酮均未刺激卵巢摄取卵黄蛋白原，但高剂量（5 mg/kg）的17β-雌二醇或睾酮处理会使性腺体指数略有增加，并导致一些初级卵母细胞闭锁。高剂量17β-雌二醇处理会使卵原细胞数量减少。
