Vissio P G, Paz D A, Maggese C
Dpto. de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Argentina.
Biocell. 1996 Aug;20(2):155-61.
The adenohypophyseal cell types of the protogynous fish Synbranchus marmoratus were studied by histochemical and immunocytochemical staining with antisera raised against piscine and human pituitary hormones to ascertain their distribution. The prolactin (PRL) cells were distributed in the rostral pars distalis and showed specific binding to antisera to carp and chum salmon prolactin. No reaction was observed with antiserum to human prolactin. The corticotrops showed strong immunoreactivity with anti-human ACTH, these cells bordered the neurohypophysis and islets between PRL cells in the rostral pars distalis. Growth hormone (GH) cells were densely distributed and associated with the neurohypophysis only in pars distalis proximal. They reacted with antisera to piscine GH but not with antisera to human growth hormone. The thyrotrops were scattered in the proximal pars distalis and showed strong immunoreactivity to the human thyrotropin Beta subunit antiserum. Gonadotrops were located in the central area of the proximal pars distalis and in the external border of the pars intermedia. These cells were alcian blue and PAS positive, and reacted with anti-croaker GTH and anti-coho GTH I and GTH II. The PAS positive cells from the pars intermedia bound specifically to anti-chum somatolactin.
通过使用针对鱼类和人类垂体激素产生的抗血清进行组织化学和免疫细胞化学染色，研究了雌性先熟的鱼类——线纹鳗鲶的腺垂体细胞类型，以确定它们的分布。催乳素（PRL）细胞分布于远侧部的前部，并且与抗鲤鱼和大麻哈鱼催乳素的抗血清有特异性结合。与抗人催乳素的抗血清没有反应。促肾上腺皮质激素细胞与抗人促肾上腺皮质激素有很强的免疫反应性，这些细胞在远侧部前部与神经垂体和PRL细胞之间的胰岛相邻。生长激素（GH）细胞密集分布，仅在远侧部近端与神经垂体相关。它们与抗鱼类GH的抗血清发生反应，但与抗人生长激素的抗血清不发生反应。促甲状腺激素细胞散布在远侧部近端，并且对人促甲状腺激素β亚基抗血清有很强的免疫反应性。促性腺激素细胞位于远侧部近端的中央区域和中间部的外缘。这些细胞阿尔辛蓝和PAS呈阳性，并且与抗黄花鱼促性腺激素、抗银大麻哈鱼促性腺激素I和促性腺激素II发生反应。来自中间部的PAS阳性细胞与抗大麻哈鱼生长抑素特异性结合。
