Füchtbauer A, Jockusch B M, Maruta H, Kilimann M W, Isenberg G
Nature. 1983;304(5924):361-4. doi: 10.1038/304361a0.
Capping proteins are F-actin binding proteins which interfere with the in vitro growth of an actin filament by blocking one of its ends (for recent reviews see refs 1-3). The majority of such proteins described so far "cap' the fast-growing (positive) end of the polar filament, thus reducing the velocity of filament growth while increasing the number of filaments being formed de novo from a monomer pool. We have studied the effects of capping proteins on the organization of actin filaments in living tissue culture cells by microinjection in conjunction with fluorescence, reflection contrast and electron microscopy. Our results, reported here, indicate that capping proteins from different sources disrupt microfilament bundles in a variety of cell types causing their disintegration from the distal end towards the centre of the cell.
封端蛋白是F-肌动蛋白结合蛋白，通过封闭肌动蛋白丝的一端来干扰其体外生长（近期综述见参考文献1-3）。到目前为止所描述的大多数此类蛋白会“封端”极性丝快速生长的（正）端，从而降低丝的生长速度，同时增加从单体库中从头形成的丝的数量。我们通过结合荧光、反射对比和电子显微镜的显微注射技术，研究了封端蛋白对活组织培养细胞中肌动蛋白丝组织的影响。我们在此报告的结果表明，来自不同来源的封端蛋白会破坏多种细胞类型中的微丝束，导致它们从细胞远端向细胞中心解体。
