Saito A, Yamamoto A, Manji T, Maeda K, Kobayashi K, Yamamoto Y, Ohta K
Contrib Nephrol. 1978;9:78-87. doi: 10.1159/000401435.
The degree of 15N incorporation into serum albumin studied by 15N-urea administration in dialyzed patients on a 1.3 g/kg/day protein diet was shown to be almost the same as in non-dialyzed uremic patients on low protein diet, while there was no incorporation in a normal subject. 14.1 g of EAA and histidine was intravenously given in dialyzed patients on the high protein diet and improvements in the level of BUN and anemia were observed. The study with 15N-leucine in a patient proved that about 36% of EAA infused during dialysis was transferred into dialysate. AAD was prescribed by giving 15-20 g of EAA, histidine and tyrosine to patients at each dialysis. The AAD enabled us to give a large amount of EAA asymptomatically in a short time, improving anemia and decreasing the BUN level. 15N-glycine administration in a dialyzed patient proved that 15N incorporation into serum albumin was 3 times greater than his non-dialyzed uremic stage on a low protein diet, and that EAA serum concentrations and non-EAA which had not been added into dialysate were elevated.
通过给接受透析且蛋白质摄入量为1.3g/kg/天的患者静脉注射15N-尿素来研究15N掺入血清白蛋白的程度，结果显示其与接受低蛋白饮食的未透析尿毒症患者几乎相同，而正常受试者中则未出现掺入情况。给接受高蛋白饮食的透析患者静脉注射14.1g必需氨基酸（EAA）和组氨酸后，观察到血尿素氮（BUN）水平和贫血症状有所改善。对一名患者进行的15N-亮氨酸研究证明，透析期间注入的EAA中约有36%转移到了透析液中。每次透析时给患者给予15 - 20g EAA、组氨酸和酪氨酸来开氨基酸透析液（AAD）处方。AAD使我们能够在短时间内无症状地给予大量EAA，改善贫血并降低BUN水平。对一名透析患者进行15N-甘氨酸给药证明，与处于低蛋白饮食的未透析尿毒症阶段相比，其血清白蛋白中15N的掺入量高出3倍，且血清EAA浓度和未添加到透析液中的非EAA均有所升高。
