Shanklin W M, Sheppard L B, Burke G W
Acta Anat (Basel). 1977;97(2):147-50. doi: 10.1159/000144728.
Air-dried rabbit blood was stained by HE, PAS and a modification of the Undritz II method. Eosin stained granules red in the eosinophil leukocytes. PAS was negative and the modified Undritz method failed to give consistent results. Cells with eosinophilic granules appeared in the corneal stroma 1 h after removing the corneal epithelium. They were stained red consistently by both eosin and the modified Undritz II method. Electron micrographs failed to demonstrate crystalloids in the granules. Because of the staining characteristics and the lack of crystalloids in their granules these cells were classified as pseudoeosinophil leukocytes. The electron micrographs showed some glycogen 12 h after denuding the cornea, however, glycogen was not well stained by PAS until 18 h after denuding.
将风干的兔血用苏木精-伊红（HE）、过碘酸希夫（PAS）染色法以及Undritz II方法的改良方法进行染色。伊红将嗜酸性粒细胞中的颗粒染成红色。PAS染色呈阴性，改良的Undritz方法未能给出一致的结果。去除角膜上皮1小时后，角膜基质中出现了带有嗜酸性颗粒的细胞。它们用伊红和改良的Undritz II方法均始终被染成红色。电子显微镜照片未能在颗粒中显示出结晶体。由于这些细胞的染色特性以及其颗粒中缺乏结晶体，因此将它们归类为假嗜酸性粒细胞。电子显微镜照片显示，在角膜剥脱12小时后可见一些糖原，然而，直到角膜剥脱18小时后，糖原才被PAS很好地染色。
