Shanklin W M, Sheppard L B, Burke G W
J Anat. 1975 Feb;119(Pt 1):97-104.
After scraping the epithelium from the rabbit's cornea, pseudoeosinophilic leucocytes appeared in the limbic area within one hour and began to advance, presumably by means of pseudopodia, into the anterior corneal stroma. The cytoplasmic granules of these cells were intensely stained by eosin and by the Undritz peroxidase method during the first hour, but were not stained by the PAS method until the eighteenth hour. Electron microscopy failed to show crystalloids in the granules. The cellular changes were followed until the fourth day, by which time most of the cells had disappeared by fragmentation, karyorrhexis and lysis.
刮除兔角膜上皮后,一小时内边缘区出现假嗜酸性白细胞,并开始通过伪足进入角膜前基质。这些细胞的胞质颗粒在最初一小时内用伊红和温德里茨过氧化物酶法染色强烈,但直到第18小时才被PAS法染色。电子显微镜检查未在颗粒中发现晶体样物质。观察细胞变化直至第四天,此时大多数细胞通过碎片化、核固缩和溶解而消失。