Rofstad E K, Brustad T
Eur J Cancer Clin Oncol. 1983 Aug;19(8):1127-31. doi: 10.1016/0277-5379(83)90038-x.
A human malignant melanoma grown in the athymic nude mouse was exposed to single doses of X-rays and tumour control (TC) was studied. The TCD50 at 70 days post-irradiation was found to be 27.4 +/- 0.6 Gy. This TCD50 is considerably lower than that predicted from the in vitro survival curve of cells from the melanoma irradiated in vivo. Studies also indicated that the tumour regrowth delay following large radiation doses possibly might be larger than indicated by the survival levels measured in vitro. Thus the radiation sensitivity of the melanoma measured in vivo appeared to be higher than that measured in vitro. This was probably not due to radiation damage to the vasculature only, as indicated by studies of the transplantability of irradiated tumours. An immune response by the nude mouse perhaps also contributed, as indicated by studies of the transplantability of the melanoma in whole-body-irradiated mice. If this was so, results from studies of the response to therapy of human tumours in the nude mouse, especially when tumour control is used as an endpoint, may not necessarily be representative for tumours in man.
将生长于无胸腺裸鼠体内的人类恶性黑色素瘤暴露于单剂量X射线下，并研究肿瘤控制情况。照射后70天的肿瘤控制剂量50%（TCD50）为27.4±0.6 Gy。该TCD50显著低于根据体内照射的黑色素瘤细胞体外存活曲线预测的值。研究还表明，大剂量辐射后的肿瘤再生长延迟可能比体外测量的存活水平所显示的更大。因此，体内测量的黑色素瘤辐射敏感性似乎高于体外测量的。这可能不仅仅是由于对脉管系统的辐射损伤，如对受照射肿瘤移植性的研究所表明的那样。如对全身照射小鼠体内黑色素瘤移植性的研究所表明的，裸鼠的免疫反应可能也有作用。如果是这样，在裸鼠体内对人类肿瘤治疗反应的研究结果，尤其是以肿瘤控制作为终点时，不一定能代表人类肿瘤的情况。
