Tenchini M L, Larrizza L, Mottura A, Colombi M, Barlati S, De Carli L
Eur J Cancer Clin Oncol. 1983 Aug;19(8):1143-9. doi: 10.1016/0277-5379(83)90040-8.
Thirty segregant clones were back-selected in 8AG or 5BUdR media from a non-tumorigenic human intraspecific hybrid line (HeLa TK- X fibroblasts HPRT-) displaying a high plasminogen activator (PA) level, a disorganized fibronectin (FN) matrix and anchorage-independence. These clones exhibited a widely modulated expression of the above markers concomitantly with different degrees of chromosome loss. Out of six representative segregant clones tested in nude mice, two were found to re-express tumorigenicity. No significant correlation was observed between PA or FN levels and anchorage-independence, as well as between these markers and tumorigenicity.
从一个非致瘤性人类种内杂交系（HeLa TK-X成纤维细胞HPRT-）中，在8AG或5-溴脱氧尿苷（5BUdR）培养基中反向选择了30个分离克隆，该杂交系表现出高纤溶酶原激活剂（PA）水平、紊乱的纤连蛋白（FN）基质和锚定非依赖性。这些克隆表现出上述标志物的广泛调节表达，同时伴有不同程度的染色体丢失。在裸鼠中测试的六个代表性分离克隆中，发现有两个重新表达了致瘤性。未观察到PA或FN水平与锚定非依赖性之间以及这些标志物与致瘤性之间存在显著相关性。
