含有人类11号/ X染色体易位的人成纤维细胞X小鼠细胞杂种不表达人纤连蛋白。

Human fibroblasts X mouse cell hybrids, containing a human 11/X translocation, do not express human fibronectin.

作者信息

Larizza L, Rampoldi E, Mottura A, Doneda L, Miggiano V, Barlati S

出版信息

Cell Biol Int Rep. 1983 May;7(5):325-32. doi: 10.1016/0309-1651(83)90072-3.

Abstract

Using a monoclonal antibody specific for human fibronectin (FN), we screened hybrid clones derived from the fusion of FN+ human fibroblasts, carrying a 11/X translocation, and FN-, HPRT- mouse cells for the production of this glycoprotein. Since no hybrid clone retaining the human der 11 chromosome was found to produce any human fibronectin, the segment of chromosome 11 included in the rearranged chromosome (11qter leads to 11p13) probably does not carry the structural locus for fibronectin.

摘要

利用一种对人纤连蛋白（FN）具有特异性的单克隆抗体，我们筛选了由携带11/X易位的FN+人成纤维细胞与FN-、HPRT-小鼠细胞融合产生的杂交克隆，以检测这种糖蛋白的产生情况。由于未发现保留人11号衍生染色体的杂交克隆产生任何人类纤连蛋白，重排染色体（11qter至11p13）中包含的11号染色体片段可能不携带纤连蛋白的结构基因座。