Tsilibary E C, Williams M C
J Histochem Cytochem. 1983 Nov;31(11):1289-97. doi: 10.1177/31.11.6684669.
Actin was identified with S1-labeling in type I and II cells, pericytes, and capillary endothelial cells in the peripheral lung of the adult rat. In type II cells abundant actin was present in the apex of cells, in microvilli, and in close association with lamellar bodies near the cell surface. Lamellar bodies secreting their content into the lumen of alveoli were always surrounded by a thick layer of actin-like material. In specimens treated with cytochalasin D the surface of type I, type II and contractile interstitial cells became irregular. In type II cells, lamellar bodies were no longer surrounded by actin-like material and no exocytic profiles of lamellar bodies were encountered. In type II cells actin filaments may be involved in moving lamellar bodies through the cytoplasm and/or in their secretion into alveoli. These observations also suggest that intact actin filaments may be required for maintenance of cell shape in various lung cells and that cells containing actin may be capable of limited contraction.
在成年大鼠外周肺的I型和II型细胞、周细胞和毛细血管内皮细胞中，肌动蛋白通过S1标记得以鉴定。在II型细胞中，丰富的肌动蛋白存在于细胞顶端、微绒毛中，并与细胞表面附近的板层小体紧密相关。将其内容物分泌到肺泡腔中的板层小体总是被一层厚厚的肌动蛋白样物质所包围。在用细胞松弛素D处理的标本中，I型、II型和收缩性间质细胞的表面变得不规则。在II型细胞中，板层小体不再被肌动蛋白样物质包围，也未遇到板层小体的胞吐轮廓。在II型细胞中，肌动蛋白丝可能参与将板层小体移动穿过细胞质和/或将其分泌到肺泡中。这些观察结果还表明，完整的肌动蛋白丝可能是维持各种肺细胞形状所必需的，并且含有肌动蛋白的细胞可能具有有限的收缩能力。
