Okabe-Kado J, Hayashi M, Honma Y, Hozumi M, Tsuruo T
Leuk Res. 1983;7(4):481-5. doi: 10.1016/0145-2126(83)90043-7.
K562/VCR cells, which are resistant to the cytotoxicity of vincristine, were isolated from human erythroleukemia K562 cells. Various compounds that induce erythroid differentiation of K562 cells were tested on K562/VCR cells. Differentiation of K562/VCR cells was not induced by actinomycin D or adriamycin alone, but the resistance of these cells to the inducers was overcome by verapamil. In contrast, mitomycin C, butyric acid and hemin induced differentiation of K562/VCR cells as effectively as that of K562 cells. These results suggest that therapy by induction of differentiation of leukemic cells is effective for leukemic cells that have acquired resistance to therapeutic drugs.
K562/VCR细胞是从人红白血病K562细胞中分离出来的，对长春新碱的细胞毒性具有抗性。在K562/VCR细胞上测试了各种诱导K562细胞红系分化的化合物。放线菌素D或阿霉素单独使用时不会诱导K562/VCR细胞分化，但维拉帕米可克服这些细胞对诱导剂的抗性。相比之下，丝裂霉素C、丁酸和血红素诱导K562/VCR细胞分化的效果与诱导K562细胞分化的效果一样好。这些结果表明，通过诱导白血病细胞分化进行治疗，对已获得治疗药物抗性的白血病细胞有效。
