Bensaude O, Babinet C, Morange M, Jacob F
Nature. 1983;305(5932):331-3. doi: 10.1038/305331a0.
In many species, the early post-fertilization development of the egg appears to occur mainly under maternal control and does not require transcription of the embryonic genome. In the mouse this situation is restricted to the one-cell stage; activation of the embryonic genome occurs at the late two-cell stage and results in a drastic change in the spectrum of proteins synthesized. This activation is preceded by a decrease in the overall synthesis of proteins at the end of the one-cell stage and the appearance, at the early two-cell stage, of a set of new polypeptides of molecular weight approximately 70,000 (70K) (refs 2, 8, 9). This can be compared with the series of events that occur after hyperthermia in differentiated cells. Heat shock results in an arrest of most transcription and translation; subsequently, expression of a limited set of genes, the heat shock genes, precedes the overall reactivation of cellular genome. Here we show that the 70K early two-cell-specific proteins are identical to two of the mouse heat shock proteins, HSP 68 and HSP 70.
在许多物种中，卵子受精后的早期发育似乎主要受母体控制，并不需要胚胎基因组进行转录。在小鼠中，这种情况仅限于单细胞阶段；胚胎基因组的激活发生在二细胞晚期，导致合成的蛋白质谱发生剧烈变化。在这种激活之前，单细胞阶段末期蛋白质的总体合成会减少，并且在二细胞早期会出现一组分子量约为70,000（70K）的新多肽（参考文献2、8、9）。这可以与分化细胞在热激后发生的一系列事件相比较。热激会导致大多数转录和翻译停止；随后，一组有限的基因即热激基因的表达先于细胞基因组的全面重新激活。我们在此表明，二细胞早期特异性的70K蛋白质与小鼠的两种热激蛋白HSP 68和HSP 70相同。
