Morange M, Diu A, Bensaude O, Babinet C
Mol Cell Biol. 1984 Apr;4(4):730-5. doi: 10.1128/mcb.4.4.730-735.1984.
In a previous paper, we have shown that in the absence of stress, mouse embryonal carcinoma cells, like mouse early embryo multipotent cells, synthesize high levels of 89- and 70-kilodalton heat shock proteins (HSP)(O. Bensaude and M. Morange, EMBO J. 2:173-177, 1983). We report here the pattern of proteins synthesized after a short period of hyperthermia in various mouse embryonal carcinoma cell lines and early mouse embryo cells. Among the various cell lines tested, two of them, PCC4-Aza R1 and PCC7-S-1009, showed an unusual response in that stimulation of HSP synthesis was not observed in these cells after hyperthermia. However, inducibility of 68- and 105-kilodalton HSP can be restored in PCC7-S-1009 cells after in vitro differentiation triggered by retinoic acid. Similarly, in the early mouse embryo, hyperthermia does not induce the synthesis of nonconstitutive HSP at the eight-cell stage, but induction of the 68-kilodalton HSP does occur at the blastocyst stage. Such a transition in the expression of HSP has already been described for Drosophila melanogaster and sea urchin embryos and recently for mouse embryos. It may be a general property of early embryonic cells.
在之前的一篇论文中，我们已经表明，在无应激状态下，小鼠胚胎癌细胞与小鼠早期胚胎多能细胞一样，会合成高水平的89千道尔顿和70千道尔顿热休克蛋白（HSP）（O. 本索德和M. 莫朗热，《欧洲分子生物学组织杂志》2:173 - 177，1983年）。我们在此报告在不同小鼠胚胎癌细胞系和小鼠早期胚胎细胞中短时间热激后蛋白质的合成模式。在所测试的各种细胞系中，其中两个，PCC4 - Aza R1和PCC7 - S - 1009，表现出异常反应，即在热激后这些细胞中未观察到HSP合成的刺激。然而，在视黄酸触发的体外分化后，PCC7 - S - 1009细胞中68千道尔顿和105千道尔顿HSP的诱导性可以恢复。同样，在小鼠早期胚胎中，热激在八细胞阶段不会诱导非组成型HSP的合成，但在囊胚阶段会发生68千道尔顿HSP的诱导。这种HSP表达的转变已经在黑腹果蝇和海胆胚胎中描述过，最近在小鼠胚胎中也有描述。它可能是早期胚胎细胞的一个普遍特性。
