Marai L, Myher J J, Kuksis A
Can J Biochem Cell Biol. 1983 Aug;61(8):840-9. doi: 10.1139/o83-107.
Natural triacylglycerols were resolved by high pressure liquid chromatography on Supelcosil LC-18 columns using a gradient of 30-90% propionitrile in acetonitrile as eluting solvent. The effluent was admitted to a quadrupole mass spectrometer via a direct liquid inlet interface. The mass spectra of the solutes were recorded in the chemical ionization mode. The triacylglycerol elution profile was obtained from the total ion current. Individual molecular species of the triacylglycerols were identified from the (MH)+ and the (MH--RCOOH)+ ions. The reversed-phase system allowed the separation of triacylglycerols on the basis of both carbon number and double-bond number, as well as of certain critical pairs of triplets of triacylglycerols with the same partition number. The method is applicable to the determination of triacylglycerol composition of both plant and animal fats. The minimum amount of sample which is required is about 50 ng in the scanning mode. However, 100 times more material must be injected in the liquid chromatograph because only 1/100th of the effluent is being injected into the mass spectrometer.
天然三酰甘油通过高压液相色谱法在Supelcosil LC - 18柱上进行分离，使用乙腈中30 - 90%的丙腈梯度作为洗脱溶剂。流出物通过直接液体进样接口进入四极杆质谱仪。溶质的质谱在化学电离模式下记录。三酰甘油的洗脱图谱由总离子流获得。三酰甘油的各个分子种类通过(MH)+和(MH - RCOOH)+离子进行鉴定。反相系统能够基于碳原子数、双键数以及具有相同分配数的某些关键三酰甘油三联体对来分离三酰甘油。该方法适用于动植物脂肪三酰甘油组成的测定。在扫描模式下所需的最小样品量约为50 ng。然而，必须在液相色谱仪中注入多100倍的物质，因为只有1/100的流出物被注入质谱仪。
