Glavin G B, Tanaka M, Tsuda A, Kohno Y, Hoaki Y, Nagasaki N
Pharmacol Biochem Behav. 1983 Aug;19(2):287-90. doi: 10.1016/0091-3057(83)90054-0.
Male Wistar rats were starved for 12 hr and then subjected to either 2 hr of wire mesh "envelope" restraint at room temperature; 2 hr of supine restraint in a specially constructed harness at room temperature or were not restrained. Eight brain regions were examined for NA level and the level of its major metabolite, MHPG-SO4. Plasma corticosterone and gastric ulcer incidence were also measured. All restrained rats displayed marked elevations in MHPG-SO4 levels in most brain regions. In addition, several brain regions in restrained animals showed a reduction in NA level. All restrained rats showed elevated plasma corticosterone levels and evidence of gastric lesions. In general, supine restraint produced greater alterations in regional brain NA turnover, greater evidence of ulcer disease, and higher plasma corticosterone levels than did wire mesh restraint. These data suggest that acute but intense stress in the form of restraint causes markedly altered brain NA activity--a possible neurochemical mechanism underlying the phenomenon of stress-induced disease.
雄性Wistar大鼠禁食12小时，然后在室温下接受2小时的铁丝网“信封”束缚；在室温下于特制的背带中仰卧束缚2小时，或者不进行束缚。检测了八个脑区的去甲肾上腺素（NA）水平及其主要代谢产物3-甲氧基-4-羟基苯乙二醇硫酸酯（MHPG-SO4）的水平。还测量了血浆皮质酮和胃溃疡发生率。所有受束缚的大鼠在大多数脑区的MHPG-SO4水平均显著升高。此外，受束缚动物的几个脑区NA水平降低。所有受束缚的大鼠血浆皮质酮水平升高，并有胃部损伤的迹象。一般来说，与铁丝网束缚相比，仰卧束缚导致脑区NA周转的变化更大，溃疡病的迹象更明显，血浆皮质酮水平更高。这些数据表明，以束缚形式出现的急性但强烈的应激会导致脑NA活性显著改变——这可能是应激诱导疾病现象背后的一种神经化学机制。
