Kraly F S, Miller L A, Gibbs J
Physiol Behav. 1983 Sep;31(3):395-9. doi: 10.1016/0031-9384(83)90208-1.
Sprague-Dawley male albino rats ate sweet milk at the midpoint of the day or night phase of a 12:12 light/dark cycle 1 min after IP 0.9% NaCl or synthetic bombesin (BBS; 2-32 micrograms/kg) following 24-hr food deprivation. Exogenous BBS inhibited food intake in a dose-related manner during the day; a linear regression line accounted for 85% of the total variance for percentage suppression of food intake by BBS in 30 min. In contrast, inhibition of eating by BBS at night was not dose-related; a linear regression line accounted for only 16% of the variance. Rats were tested under identical conditions following 3-hr food deprivation. Exogenous BBS (4-64 micrograms/kg) inhibited food intake in a dose-related manner at night; a linear regression line accounted for 92% of the total variance. In contrast, inhibition of eating by BBS during the day was not as orderly; a linear regression line accounted for only 44% of the variance. The 8 micrograms/kg dose was twice as potent for inhibition of eating of 25% GIBCO 116EC liquid diet than it was for inhibition of eating sweet milk at the midpoint of the day phase. These results describe diurnal variation in potency of exogenous BBS for inhibition of food intake in the rat. Whether BBS is more or less potent at night than during the day depends upon the particular dose, degree of hunger of the rat, and probably the type of food being eaten.
斯普拉格-道利雄性白化大鼠在12:12光/暗循环的白天或黑夜阶段的中点，于腹腔注射0.9%氯化钠或合成蛙皮素（BBS；2 - 32微克/千克）1分钟后，在24小时食物剥夺后进食甜牛奶。外源性BBS在白天以剂量相关的方式抑制食物摄入；一条线性回归线占BBS在30分钟内抑制食物摄入百分比总方差的85%。相比之下，BBS在夜间对进食的抑制与剂量无关；一条线性回归线仅占方差的16%。在3小时食物剥夺后，大鼠在相同条件下接受测试。外源性BBS（4 - 64微克/千克）在夜间以剂量相关的方式抑制食物摄入；一条线性回归线占总方差的92%。相比之下，BBS在白天对进食的抑制不太规律；一条线性回归线仅占方差的44%。8微克/千克剂量对抑制25% GIBCO 116EC液体饮食进食的效力是其在白天中点抑制进食甜牛奶效力的两倍。这些结果描述了外源性BBS抑制大鼠食物摄入效力的昼夜变化。BBS在夜间比白天更有效还是相反，取决于特定剂量、大鼠的饥饿程度以及可能所吃食物的类型。
