Bei S Z, Li Y A, Li L, Pan Z Y, Li G X, Lan B X, Bao Y H, Yan Q Y, Wang Q
Sci Sin B. 1983 Jul;26(7):708-15.
Chromatin prepared both from the yolk granules beneath the blastoderm and from the nuclei of the blastoderm cells of fertilized and unincubated chicken eggs was examined under electron microscope and its structure was compared. The results indicate that the above two kinds of chromatin fibers seem to have no apparent difference in structural relation and diameter. The electron microscopic appearances of the basic structures of both kinds of chromatin are all "beads-on-a-string"-shaped filaments. After the digestion of chromatin from both raw materials with DNase I, the connecting strings of chromatin fibers were cut off, only the scattered beads could be observed under electron microscope. The diameter of the scattered beads is equal to that of the beads on the chromatin fibers, and most of them are 150 A in diameter.
对从孵化前受精鸡蛋的胚盘下卵黄颗粒以及胚盘细胞核制备的染色质进行了电子显微镜检查,并比较了它们的结构。结果表明,上述两种染色质纤维在结构关系和直径上似乎没有明显差异。两种染色质基本结构的电子显微镜外观均为“串珠”状细丝。用DNase I消化这两种原料的染色质后,染色质纤维的连接串被切断,电子显微镜下只能观察到分散的珠子。分散珠子的直径与染色质纤维上珠子的直径相等,大多数直径为150埃。