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受精未孵化鸡蛋胚盘细胞核及胚盘下卵黄颗粒中DNA分子的电子显微镜观察。

Electron microscopy of DNA molecules from blastoderm nuclei and yolk granules beneath blastoderm of fertilized and unincubated chicken eggs.

作者信息

Bei S Z, Li Y A, Li L, Pan Z Y, Wang Q, Yan Q Y

出版信息

Sci Sin B. 1983 Aug;26(8):818-22.

PMID:6684789
Abstract

The DNA molecules isolated from the blastoderm nuclei and yolk granules beneath the blastoderm of fertilized and unincubated chicken eggs were observed under electron microscope, using the DNA sample of calf thymus as control. The results showed that both nuclear and yolk DNAs are linear, and their molecules are very similar to those of thymus DNA in shape. Since chromatin was first prepared from blastoderm nuclei and yolk granules respectively and then DNA was isolated from both of chromatin samples, it could be concluded that yolk granules possess both DNA chromatin as nuclei do.

摘要

以小牛胸腺DNA样品为对照,在电子显微镜下观察从受精未孵化鸡蛋的胚盘细胞核和胚盘下卵黄颗粒中分离出的DNA分子。结果表明,细胞核DNA和卵黄DNA均为线性,其分子形状与胸腺DNA的分子形状非常相似。由于分别从胚盘细胞核和卵黄颗粒中制备染色质,然后从这两种染色质样品中分离DNA,因此可以得出结论,卵黄颗粒与细胞核一样都具有DNA染色质。

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