用各种细胞毒素对狗进行离体肝脏灌注后肝细胞的超微结构变化。

Ultrastructural changes in the dog liver cell after isolated liver perfusion with various cytotoxins.

作者信息

Merker G, Helling H J, Krahl M, Aigner K

出版信息

Recent Results Cancer Res. 1983;86:103-9. doi: 10.1007/978-3-642-82025-0_19.

DOI:10.1007/978-3-642-82025-0_19

Abstract

Following isolated liver perfusion with different cytotoxins - 5-fluorouracil (5-FU), methotrexate (MTX), dacarbazine (DTIC) cis-platinum (cis-PT) - liver tissue of dogs was examined with the electron microscope (a) directly after the perfusion and (b) after a survival time of 4 weeks (5-FU and DTIC). Acute disintegration of the granular endoplasmic reticulum and depletion of the glycogen stores occurred after perfusion with 5-FU, MTX, and DTIC, while cis-PT induced disintegration of the granular endoplasmic reticulum accompanied by an accumulation of glycogen. Four weeks after perfusion with DTIC signs of remarkably increased cellular activity were observed, while 4 weeks after perfusion with 5-FU the parenchymal liver cells revealed a well-balanced and moderate recovery of the cellular structures.

摘要

在用不同的细胞毒素——5-氟尿嘧啶（5-FU）、甲氨蝶呤（MTX）、达卡巴嗪（DTIC）、顺铂（cis-PT）进行离体肝脏灌注后，对犬的肝脏组织进行了电子显微镜检查：（a）灌注后立即检查，（b）在存活4周后检查（5-FU和DTIC组）。用5-FU、MTX和DTIC灌注后，粗面内质网急性崩解，糖原储备耗竭，而顺铂诱导粗面内质网崩解并伴有糖原积累。用DTIC灌注4周后，观察到细胞活性显著增加的迹象，而用5-FU灌注4周后，肝实质细胞显示出细胞结构的平衡且适度的恢复。