Gaffney P J, Templeman J, Curtis A D, Campbell P J
Thromb Haemost. 1983 Oct 31;50(3):650-1.
Since tissue-type plasminogen activator (t-PA) derived from tissue culture and recombinant DNA procedures has been proposed for use in thrombolytic therapy in man, it is essential that the t-PA molecule should display reasonable stability in a lyophilised state to facilitate its usefulness. In this study, four laboratories compared the potencies of three preparations of t-PA following storage at 4 degrees, 20 degrees, 37 degrees and 45 degrees C, using each t-PA stored at -20 degrees C as a reference (100% activity) in each case. A pig heart extract of t-PA was the most stable, losing no activity when stored for 30 days at 37 degrees C, while two melanoma cell tissue culture extracts varied in their storage behaviour. One was quite stable at 37 degrees C (losing about 3% of its activity) while the other lost about 16%. Thus both the pig heart t-PA and one t-PA from melanoma cell culture proved suitable for the further development of reference standards for t-PA activity.
由于源自组织培养和重组DNA程序的组织型纤溶酶原激活剂（t-PA）已被提议用于人类的溶栓治疗，因此t-PA分子在冻干状态下应具有合理的稳定性以方便其使用，这一点至关重要。在本研究中，四个实验室比较了三种t-PA制剂在4℃、20℃、37℃和45℃储存后的效价，每种情况下均将储存在-20℃的t-PA用作参考（100%活性）。猪心t-PA提取物最稳定，在37℃储存30天后无活性损失，而两种黑色素瘤细胞组织培养提取物的储存行为有所不同。一种在37℃相当稳定（活性损失约3%），而另一种损失约16%。因此，猪心t-PA和一种来自黑色素瘤细胞培养的t-PA都被证明适用于进一步开发t-PA活性参考标准。
