Shibusawa H, Sano Y, Yamamoto T, Kambegawa A, Ohkawa T, Satoh N, Okinaga S, Arai K
Endocrinol Jpn. 1978 Apr;25(2):185-9. doi: 10.1507/endocrj1954.25.185.
A radioimmunoassay for plasma 16alpha-hydroxypregnenolone has been developed using anti-16alpha-hydroxypregnenolone antiserum produced in rabbits immunized with 16 alpha-hydroxypregnenolone-3-succinate-bovine serum albumin. Radioimmunoassay data were processed and analysed for its validity using a four parameter logistic curve fitting routine with use of the IBM call system. The assay system was satisfactory for a routine use. The 16alpha-hydroxypregnenolone concentrations in the umbilical cord blood at term ranged from 223 to 324 ng/ml. Gas liquid chromatographic analysis revealed a similar result, and also the presence of this steroid in the adrenal gland of the human fetus at midtrimester was confirmed. It is speculated that this steroid is secreted from fetal adrenals.
已开发出一种用于检测血浆16α-羟基孕烯醇酮的放射免疫分析法，该方法使用的抗血清是由用16α-羟基孕烯醇酮-3-琥珀酸-牛血清白蛋白免疫的兔子产生的抗16α-羟基孕烯醇酮抗血清。利用IBM调用系统的四参数逻辑曲线拟合程序对放射免疫分析数据进行处理和有效性分析。该检测系统适合常规使用。足月时脐带血中16α-羟基孕烯醇酮的浓度范围为223至324 ng/ml。气相色谱分析得出了类似结果，并且证实了在孕中期人类胎儿的肾上腺中存在这种类固醇。据推测，这种类固醇是由胎儿肾上腺分泌的。
