Törmä H, Vahlquist A
Arch Dermatol Res. 1983;275(5):324-8. doi: 10.1007/BF00417206.
In connection with studies on vitamin A transport to keratinocytes in vivo, the retinol, retinol-binding protein (RBP), and prealbumin contents of blister fluid obtained subepidermally (suction) or intraepidermally (friction) were analyzed. The median concentrations of the three components in suction blister fluid (SBF) were all about 33% of the serum concentrations. Corresponding values for alpha 2-macroglobulin and total protein, measured as controls, were 27 and 30%, respectively. The relative amounts of retinol and prealbumin in friction blister fluid (FBF) were the same as those in SBF. By contrast, FBF contained more RBP (median value 40%; P less than 0.01 vs SBF value). It is suggested that apo-RBP (RBP without retinol) is generated within the epidermis and contributes to the high RBP value in FBF. FBF constituents are probably derived from the intercellular space of the epidermis, at least during the initial phase of blister formation. Accordingly, RBP and prealbumin were identified in homogenates of pure epidermis. During the later stages of blister formation, FBF resembles an ultrafiltrate of dermal fluid, although different in this respect from SBF.
在关于维生素A向体内角质形成细胞转运的研究中，分析了通过皮下（抽吸）或表皮内（摩擦）获取的水疱液中的视黄醇、视黄醇结合蛋白（RBP）和前白蛋白含量。抽吸水疱液（SBF）中这三种成分的中位浓度均约为血清浓度的33%。作为对照测量的α2-巨球蛋白和总蛋白的相应值分别为27%和30%。摩擦水疱液（FBF）中视黄醇和前白蛋白的相对含量与SBF中的相同。相比之下，FBF含有更多的RBP（中位值40%；与SBF值相比，P<0.01）。提示脱辅基RBP（不含视黄醇的RBP）在表皮内产生，并导致FBF中RBP值较高。FBF成分可能至少在水疱形成的初始阶段来源于表皮的细胞间隙。因此，在纯表皮匀浆中鉴定出了RBP和前白蛋白。在水疱形成的后期，FBF类似于真皮液的超滤液，尽管在这方面与SBF不同。
