Stephan W, Dichtelmüller H
Arzneimittelforschung. 1983;33(11):1538-40.
Two intravenous 7S-immunoglobulin (IgG) preparations, beta-propiolactone(beta-PL)-treated (Intraglobin) and hydrochloric acid/pepsin-treated, were tested for their in vitro behaviour in haemagglutination, anticomplementary activity and binding to SPA-sepharose. These results were compared with the efficacy of these preparations in vivo, using mouse protection tests with Salmonella and Pseudomonas as a challenge. In spite of marked differences in the in vitro behaviour, both preparations showed almost the same efficacy in vivo. These results indicate that in vitro findings do not allow predictions of the in vivo efficacy of intravenous IgG preparations.
对两种静脉注射用7S免疫球蛋白（IgG）制剂进行了测试，一种是经β-丙内酯（β-PL）处理的（Intraglobin），另一种是经盐酸/胃蛋白酶处理的，检测它们在血凝、抗补体活性以及与葡萄球菌A蛋白-琼脂糖（SPA-琼脂糖）结合方面的体外行为。将这些结果与这些制剂在体内的功效进行了比较，采用以沙门氏菌和假单胞菌为攻击菌的小鼠保护试验。尽管体外行为存在显著差异，但两种制剂在体内显示出几乎相同的功效。这些结果表明，体外研究结果无法预测静脉注射IgG制剂的体内功效。
