Ceuterick F, Peeters J, Heremans K, De Smedt H, Olbrechts H
Eur J Biochem. 1978 Jun 15;87(2):401-7. doi: 10.1111/j.1432-1033.1978.tb12389.x.
It is shown that lipids are responsible for the breaks in the Arrhenius plots of Azotobacter nitrogenase. The physical evidence is that temperature at which the break occurs increases with increasing pressure by 20 K/1000 atm. This is in agreement with the pressure dependence of the transition temperature of several synthetic phospholipids. We also find the same pressure dependence for the broad transitions observed in Escherichia coli phosphatidylethanolamine and in the membrane lipids from Azotobacter itself. Detergents and phospholipase remove the break. Reconstruction can be performed only with specific phospholipids.
结果表明，脂质是导致固氮菌固氮酶阿累尼乌斯曲线出现断点的原因。物理证据是，断点出现时的温度随压力升高而升高，变化速率为20 K/1000 atm。这与几种合成磷脂转变温度的压力依赖性一致。我们还发现，在大肠杆菌磷脂酰乙醇胺和固氮菌自身的膜脂中观察到的宽泛转变也具有相同的压力依赖性。去污剂和磷脂酶可消除断点。只有特定的磷脂才能进行重构。
