肼是由褐球固氮菌的钒固氮酶将二氮还原产生的产物。
Hydrazine is a product of dinitrogen reduction by the vanadium-nitrogenase from Azotobacter chroococcum.
作者信息
Dilworth M J, Eady R R
机构信息
School of Biological and Environmental Sciences, Murdoch University, Western Australia.
出版信息
Biochem J. 1991 Jul 15;277 ( Pt 2)(Pt 2):465-8. doi: 10.1042/bj2770465.
Abstract
During the enzymic reduction of N2 to NH3 by Mo-nitrogenase, free hydrazine (N2H4) is not detectable, but an enzyme-bound intermediate can be made to yield N2H4 by quenching the enzyme during turnover [Thorneley, Eady & Lowe (1978) Nature (London) 272, 557-558]. In contrast, we show here that the V-nitrogenase of Azotobacter chroococcum produces a small but significant amount of free N2H4 (up to 0.5% of the electron flux resulting in N2 reduction) as a product of the reduction of N2. The amount of N2H4 formed increased 15-fold on increasing the assay temperature from 20 degrees C to 40 degrees C. Activity cross-reactions between nitrogenase components of Mo- and V-nitrogenases showed that the formation of free N2H4 was associated with the VFe protein. These data provide the first direct evidence for an enzyme intermediate at the four-electron-reduced level during the reduction of N2 by V-nitrogenase.
摘要
在钼固氮酶将N₂酶促还原为NH₃的过程中，未检测到游离肼（N₂H₄），但在周转过程中通过淬灭酶，一种与酶结合的中间体可产生N₂H₄[索恩利、伊迪和洛（1978年），《自然》（伦敦）272, 557 - 558]。相比之下，我们在此表明，褐球固氮菌的钒固氮酶会产生少量但显著量的游离N₂H₄（高达导致N₂还原的电子通量的0.5%），作为N₂还原的产物。将测定温度从20℃提高到40℃时，形成的N₂H₄量增加了15倍。钼固氮酶和钒固氮酶的固氮酶组分之间的活性交叉反应表明，游离N₂H₄的形成与VFe蛋白有关。这些数据为钒固氮酶将N₂还原过程中处于四电子还原水平的酶中间体提供了首个直接证据。
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Hydrazine and biological nitrogen fixation.肼与生物固氮
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Molybdenum and vanadium nitrogenases of Azotobacter chroococcum. Low temperature favours N2 reduction by vanadium nitrogenase.褐球固氮菌的钼和钒固氮酶。低温有利于钒固氮酶进行N2还原。
Biochem J. 1988 Dec 1;256(2):429-32. doi: 10.1042/bj2560429.
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The vanadium nitrogenase of Azotobacter chroococcum. Reduction of acetylene and ethylene to ethane.褐球固氮菌的钒固氮酶。乙炔和乙烯还原为乙烷。
Biochem J. 1988 Feb 1;249(3):745-51. doi: 10.1042/bj2490745.
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Isolation of a new vanadium-containing nitrogenase from Azotobacter vinelandii.从棕色固氮菌中分离出一种新的含钒固氮酶。
Biochemistry. 1986 Nov 18;25(23):7251-5. doi: 10.1021/bi00371a001.
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The vanadium nitrogenase of Azotobacter chroococcum. Purification and properties of the Fe protein.褐球固氮菌的钒固氮酶。铁蛋白的纯化及性质
Biochem J. 1988 Nov 15;256(1):189-96. doi: 10.1042/bj2560189.
10
The vanadium-iron protein of vanadium nitrogenase from Azotobacter chroococcum contains an iron-vanadium cofactor.来自褐球固氮菌的钒固氮酶的钒铁蛋白含有一个铁钒辅因子。
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