氨对盘基网柄菌浸没单层中柄细胞形成的影响。

The effect of ammonia on stalk cell formation in submerged monolayers of Dictyostelium discoideum.

作者信息

Neave N, Sobolewski A, Weeks G

出版信息

Cell Differ. 1983 Dec;13(4):301-7. doi: 10.1016/0045-6039(83)90040-4.

Abstract

It has been shown that ammonia inhibits stalk cell formation in monolayers of V12M2, and it was suggested that this inhibition was due to an antagonism of the differentiation-inducing factor (DIF) (Gross, J.D. et al., Nature, 303, 244-246, 1983). However, the results presented here indicate that ammonia inhibition is independent of DIF concentration, and that it occurs well in advance of the period of DIF requirement. Ammonia completely inhibits DIF accumulation and inhibits stalk cell differentiation, but there is no inhibition of prespore cell formation. These results imply the existence of an early ammonia-sensitive event that influences terminal cell type differentiation.

摘要

研究表明，氨会抑制V12M2单层细胞中柄细胞的形成，有人认为这种抑制是由于分化诱导因子（DIF）的拮抗作用（格罗斯，J.D.等人，《自然》，第303卷，第244 - 246页，1983年）。然而，此处给出的结果表明，氨的抑制作用与DIF浓度无关，且在需要DIF的时期之前就已充分发生。氨完全抑制DIF的积累并抑制柄细胞分化，但对前孢子细胞的形成没有抑制作用。这些结果意味着存在一个早期的氨敏感事件，它会影响终末细胞类型的分化。