Iyengar A K, Narasinga Rao B S
Hum Nutr Clin Nutr. 1983 Jan;37(1):43-51.
A long-term N balance study was carried out in five preschool children 3-4 years of age fed a cereal-legume-milk diet providing 1.75 g protein and 100 kcal (418 kJ) energy/kg body weight. This level of protein was found to be safe for preschool children in an earlier short-term N balance study. Continuous N balance was determined for 70 d in three subjects and 45 d in two subjects. All the subjects were found to be in steady positive N balance throughout the experimental period, the mean daily N retention varying between 54.5 and 87.5 mg/d. All the children increased their body weight steadily over the experimental period. No tendency of N balance to decrease with time was observed. It is concluded that the safe level of protein for preschool children estimated from a short-term N balance study is quite adequate when tested by a long-term feeding trial.
对5名3 - 4岁的学龄前儿童进行了一项长期氮平衡研究，这些儿童食用谷类 - 豆类 - 奶类饮食，每千克体重摄入1.75克蛋白质和100千卡（418千焦）能量。在早期的一项短期氮平衡研究中发现，这个蛋白质水平对学龄前儿童是安全的。对3名受试者持续测定氮平衡70天，对2名受试者测定45天。在整个实验期间，所有受试者均处于稳定的正氮平衡状态，每日平均氮潴留量在54.5至87.5毫克/天之间。在实验期间，所有儿童体重均稳步增加。未观察到氮平衡随时间下降的趋势。得出结论：通过长期喂养试验测试时，从短期氮平衡研究中估算出的学龄前儿童蛋白质安全水平是相当充足的。
