酶联免疫吸附测定法检测海曼抗原，该抗原是致肾炎糖肽、致肾炎糖苷中的一种污染性次要成分。
Enzyme linked immunosorbent assay for Heymann's antigen as a contaminating minor component in nephritogenic glycopeptide, nephritogenoside.
作者信息
Natori Y, Shibata S
出版信息
Clin Exp Immunol. 1983 Mar;51(3):595-9.
An enzyme linked immunosorbent assay (ELISA) was introduced for the detection of nephritogenic glycopeptide, nephritogenoside and Heymann's antigen. In the fraction of crude nephritogenoside which induced membranous glomerulonephritis (besides proliferative glomerulonephritis) in rats, both nephritogenoside and Heymann's antigen were detected by ELISA. On the other hand, in the sample of pure nephritogenoside which induced only proliferative glomerulonephritis, Heymann's antigen was not detected at all. These results indicate that crude nephritogenoside preparation contains Heymann's antigen as an inducing factor of membranous glomerulonephritis in homologous animals. In addition to TCA treatment or DEAE-cellulose column chromatography, gel filtration on Bio-Gel P200 was a good tool for the removal of Heymann's antigen from nephritogenoside.
采用酶联免疫吸附测定法（ELISA）检测致肾炎糖肽、致肾炎糖苷和海曼抗原。在诱导大鼠膜性肾小球肾炎（除增生性肾小球肾炎外）的粗制致肾炎糖苷组分中，ELISA检测到了致肾炎糖苷和海曼抗原。另一方面，在仅诱导增生性肾小球肾炎的纯致肾炎糖苷样本中，完全未检测到海曼抗原。这些结果表明，粗制致肾炎糖苷制剂含有海曼抗原，作为同源动物膜性肾小球肾炎的诱导因子。除了三氯乙酸处理或二乙氨基乙基纤维素柱色谱法外，使用Bio-Gel P200进行凝胶过滤是从致肾炎糖苷中去除海曼抗原的良好方法。
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