Shibata S, Natori Y, Hayakawa I
Clinical Research Institute, National Medical Center, Tokyo, Japan.
Nephron. 1989;51(2):243-9. doi: 10.1159/000185293.
Studies on the immunochemical characterization were made on the antigenic substance of membranous glomerulonephritis (MG) that is induced, in homologous animals, by a single footpad injection of rat crude nephritogenoside (CNG). In rats injected with rat CNG, typical MG with immunofluorescent granular pattern (immune complex type nephritis) was produced 3-4 months after the injection. Since CNG nephritis is a model which induces MG only in homologous animals, the development of MG in this model should be explained by antigen-autoantibody immune complex mechanism. Thus, the features of the antigenic substance contained in CNG was immunochemically investigated, utilizing the serum (autoantibody) of rats with MG as well as rabbit antiserum to rat tubular brush border antigen (FXIA) (heteroantibody). As a result, two different fractions (fr. 31 and fr. 36) were separated from the antigenic substance contained in CNG: that is, fr. 31 reacts only with rabbit antiserum to rat tubular brush border antigen (FXIA) (heteroantibody) but does not react with the serum (autoantibody) of rats with MG. Fr. 36 does not react with rabbit antiserum to FXIA (heteroantibody) and reacts only with the serum (autoantibody) of rats with MG (which was induced by a single footpad injection of CNG or soluble FXIA). These results suggest immunologically that fr. 36 is the main antigen responsible for the development of MG which is induced in homologous animals by a single footpad injection of CNG.
对膜性肾小球肾炎（MG）的抗原物质进行了免疫化学特性研究。MG是通过在同源动物的单足垫注射大鼠粗制肾炎原糖苷（CNG）诱导产生的。在注射大鼠CNG的大鼠中，注射后3 - 4个月产生了具有免疫荧光颗粒模式的典型MG（免疫复合物型肾炎）。由于CNG肾炎是仅在同源动物中诱导MG的模型，该模型中MG的发生应通过抗原 - 自身抗体免疫复合物机制来解释。因此，利用MG大鼠的血清（自身抗体）以及兔抗大鼠肾小管刷状缘抗原（FXIA）的抗血清（异种抗体），对CNG中所含抗原物质的特性进行了免疫化学研究。结果，从CNG中所含的抗原物质中分离出了两种不同的组分（组分31和组分36）：即，组分31仅与兔抗大鼠肾小管刷状缘抗原（FXIA）的抗血清（异种抗体）反应，但不与MG大鼠的血清（自身抗体）反应。组分36不与抗FXIA的兔抗血清（异种抗体）反应，仅与MG大鼠的血清（自身抗体）反应（通过单足垫注射CNG或可溶性FXIA诱导）。这些结果在免疫学上表明，组分36是通过单足垫注射CNG在同源动物中诱导产生MG的主要抗原。
