利用从牛凝血因子IX氨基酸序列推导出来的一段独特的52个碱基的合成寡核苷酸探针分离人抗血友病因子IX cDNA克隆。
Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX.
作者信息
Jaye M, de la Salle H, Schamber F, Balland A, Kohli V, Findeli A, Tolstoshev P, Lecocq J P
出版信息
Nucleic Acids Res. 1983 Apr 25;11(8):2325-35. doi: 10.1093/nar/11.8.2325.
Abstract
A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisely one codon has been deleted.Images
摘要
根据牛凝血因子IX的氨基酸序列推导合成了一种独特的52聚体寡核苷酸，并将其用作探针筛选人肝脏cDNA文库。与先前分离的克隆相比，分离得到的凝血因子IX克隆在核苷酸和推导的氨基酸序列上有5处差异。此外，恰好缺失了一个密码子。
图像
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