编码牛纤维蛋白原β链的cDNA克隆的特性分析
Characterization of a cDNA clone coding for the beta chain of bovine fibrinogen.
作者信息
Chung D W, Rixon M W, MacGillivray R T, Davie E W
出版信息
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1466-70. doi: 10.1073/pnas.78.3.1466.
Abstract
Recombinant plasmids containing bovine cDNA have been screened with a radiolabeled cDNA enriched for bovine fibrinogen. A number of plasmids containing cDNAs for fibrinogen were identified by this assay. One plasmid, designated pBI beta 1, was found to contain a cDNA insert of 1372 base pairs. The sequence of the cDNA insert for this plasmid was then determined. It was shown to code for 424 amino acids of the beta chain of fibrinogen, starting with residue 44. This and other data made it possible to construct the complete amino acid sequence of the beta chain of the protein. Comparison of the amino acid sequence of the beta chain of bovine fibrinogen with the corresponding chain of the human molecule indicated that the two chains are greater than 80% homologous.
摘要
已用富含牛纤维蛋白原的放射性标记cDNA筛选了含牛cDNA的重组质粒。通过该检测鉴定出了许多含纤维蛋白原cDNA的质粒。发现一个名为pBI beta 1的质粒含有一个1372个碱基对的cDNA插入片段。然后确定了该质粒cDNA插入片段的序列。结果表明，它编码纤维蛋白原β链的424个氨基酸，从第44位残基开始。这些数据以及其他数据使得构建该蛋白质β链的完整氨基酸序列成为可能。牛纤维蛋白原β链的氨基酸序列与人类分子相应链的比较表明，这两条链的同源性大于80%。
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