从离体灌流大鼠心脏中释放嘌呤核苷和氧嘌呤

Release of purine nucleosides and oxypurines from the isolated perfused rat heart.

作者信息

de Jong J W, Harmsen E, de Tombe P P, Keijzer E

出版信息

Adv Myocardiol. 1983;4:339-45. doi: 10.1007/978-1-4757-4441-5_31.

Abstract

In the ischemic heart, high-energy phosphates are rapidly broken down. We studied the release of AMP catabolites from the isolated perfused rat heart which was temporarily made ischemic or anoxic. We measured the concentration of purine nucleosides and oxypurines with a novel high-pressure liquid chromatographic technique. The postischemic working heart released adenosine, inosine, hypoxanthine, and also substantial amounts of xanthine. The latter could indicate that xanthine oxidase is present in rat heart. Further evidence for the myocardial occurrence of this enzyme was obtained from experiments with hearts perfused retrogradely with allopurinol, an inhibitor of xanthine oxidase. This drug greatly enhanced the release of hypoxanthine, both during normoxic and anoxic perfusions. We conclude that xanthine oxidase could play an essential role in the myocardial breakdown of AMP catabolites.

摘要

在缺血性心脏中，高能磷酸盐会迅速分解。我们研究了从暂时缺血或缺氧的离体灌注大鼠心脏中AMP分解代谢产物的释放情况。我们使用一种新型的高压液相色谱技术测量了嘌呤核苷和氧嘌呤的浓度。缺血后工作的心脏释放出腺苷、肌苷、次黄嘌呤，还有大量的黄嘌呤。后者可能表明大鼠心脏中存在黄嘌呤氧化酶。通过用黄嘌呤氧化酶抑制剂别嘌呤醇逆行灌注心脏的实验，获得了该酶在心肌中存在的进一步证据。这种药物在常氧灌注和缺氧灌注期间都大大增加了次黄嘌呤的释放。我们得出结论，黄嘌呤氧化酶可能在AMP分解代谢产物的心肌分解中起重要作用。