Ronca-Testoni S, Borghini F
Ital J Biochem. 1982 Mar-Apr;31(2):127-38.
The release of purine compounds from the perfused rat heart under basal conditions was determined by high pressure liquid chromatography. Uric acid resulted the major degradative released into the perfusate. Lower levels of hypoxanthine, xanthine and inosine were found. The uric acid concentration showing that the rat heart is able to catabolize the purine compounds up to uric acid. No leakage of catabolic enzymes was observed and thereby the breakdown of the released nucleosides and bases proved to be intracellular. This heart ability was confirmed by the analysis of the degradation products of AMP added to the perfusion medium in the recirculating system. AMP was sequentially broken down to adenosine, then to inosine, hypoxanthine and xanthine and finally to uric acid that as end product accumulated in the perfusate.
在基础条件下，通过高压液相色谱法测定灌注大鼠心脏中嘌呤化合物的释放情况。尿酸是灌注液中释放的主要降解产物。还发现了较低水平的次黄嘌呤、黄嘌呤和肌苷。尿酸浓度表明大鼠心脏能够将嘌呤化合物分解代谢为尿酸。未观察到分解代谢酶的泄漏，因此所释放的核苷和碱基的分解被证明是细胞内的。通过对再循环系统中添加到灌注培养基中的AMP降解产物的分析，证实了心脏的这种能力。AMP依次分解为腺苷，然后分解为肌苷、次黄嘌呤和黄嘌呤，最后分解为尿酸，尿酸作为终产物在灌注液中积累。
