非洲爪蟾白蛋白信使核糖核酸互补脱氧核糖核酸的合成与特性分析

Synthesis and characterization of a DNA complementary to Xenopus laevis albumin mRNA.

作者信息

Dimitriadis G J, Zongza-Dimitriadis V

出版信息

Mol Cell Biochem. 1983;52(2):139-44. doi: 10.1007/BF00224922.

Abstract

Albumin complementary DNA (cDNA) was transcribed from purified albumin mRNA from the liver of Xenopus laevis. The resultant cDNA was an almost full length copy as defined by denaturing gel electrophoresis; was hybridized specifically to albumin mRNA with pseudo-first-order reaction kinetics and the mRNA . cDNA hybrid exhibited a sharp melting profile with a Tm of 83 degrees C. The identity of the cDNA was confirmed by gel electrophoresis following hybridization-arrested translation.

摘要

白蛋白互补脱氧核糖核酸（cDNA）是从非洲爪蟾肝脏中纯化的白蛋白信使核糖核酸（mRNA）转录而来。所得到的cDNA经变性凝胶电泳鉴定几乎是全长拷贝；以拟一级反应动力学与白蛋白mRNA特异性杂交，且mRNA-cDNA杂交体呈现出尖锐的解链曲线，解链温度为83摄氏度。通过杂交抑制翻译后的凝胶电泳证实了该cDNA的一致性。