May F E, Ryffel G U, Weber R, Westley B R
J Biol Chem. 1982 Dec 10;257(23):13919-23.
The levels of albumin mRNA in Xenopus laevis liver were measured at various times after injection of estradiol using two different methods involving hybridization of cloned albumin cDNA to total liver RNA. The absolute levels of albumin mRNA fell by more than 95% during the first 4 days following estrogen treatment, then slowly returned to normal levels over the following 12 days. Albumin synthesis paralleled the albumin mRNA levels during the first 8 days after injection; but, 16 and 32 days after injection, albumin synthesis again decreased while albumin mRNA remained at normal levels. The time courses of the effects of estrogen on albumin and vitellogenin mRNA levels were different. Whereas albumin mRNA levels were minimal 4 days after estradiol injection, vitellogenin mRNA levels were maximal 8 days after injection.
使用两种不同方法，即通过克隆的白蛋白cDNA与肝脏总RNA杂交，在注射雌二醇后的不同时间测量非洲爪蟾肝脏中白蛋白mRNA的水平。雌激素处理后的前4天，白蛋白mRNA的绝对水平下降超过95%，随后在接下来的12天里缓慢恢复到正常水平。注射后的前8天，白蛋白合成与白蛋白mRNA水平平行；但是，注射后16天和32天，白蛋白合成再次下降，而白蛋白mRNA仍维持在正常水平。雌激素对白蛋白和卵黄蛋白原mRNA水平影响的时间进程不同。雌二醇注射后4天白蛋白mRNA水平最低，而卵黄蛋白原mRNA水平在注射后8天最高。
