Chiovato L, Hammond L J, Hanafusa T, Pujol-Borrell R, Doniach D, Bottazzo G F
Clin Endocrinol (Oxf). 1983 Nov;19(5):581-90. doi: 10.1111/j.1365-2265.1983.tb00034.x.
A new bioassay is described for detecting the growth stimulating immunoglobulins (TGI) that contribute to goitre formation in human thyroid autoimmune diseases. It measures the incorporation of tritiated thymidine into intact rat thyroid follicles grown in tissue culture. This radiometric assay demands much less technical skill than the cytochemical bioassays (CBA) previously employed. It has good reproducibility and the techniques and apparatus are available in many clinical laboratories. Immunoglobulins (Igs) from 68% of patients with goitrous Graves' disease were positive, in proportion with goitre size, and this showed no correlation with T3 levels, or three accepted methods for conventional thyroid stimulating antibodies. Non-toxic nodular goitre cases gave positive results in 3/9 who had recurrences after one or more thyroidectomies and in 1/10 cases of familial simple goitre. All normal subjects and all endemic goitre cases were negative as well as 21 cases of sporadic non-toxic nodular goitre. Although it is less sensitive than the 'growth CBA' it clearly emphasizes the essential difference between the intensity of growth stimulus which leads to the regular hyperplasia of thyroid epithelium seen in Graves' thyrotoxicosis and the disorganized and metabolically uncoordinated hyperplasia typical of non-toxic nodular goitre.
本文描述了一种新的生物测定法，用于检测在人类甲状腺自身免疫性疾病中导致甲状腺肿形成的生长刺激免疫球蛋白（TGI）。该方法通过测量氚标记的胸腺嘧啶核苷掺入组织培养中生长的完整大鼠甲状腺滤泡的情况来进行检测。与先前使用的细胞化学生物测定法（CBA）相比，这种放射性测定法所需的技术技能要少得多。它具有良好的重复性，并且许多临床实验室都具备相关技术和设备。68%的甲状腺肿性格雷夫斯病患者的免疫球蛋白（Igs）呈阳性，且与甲状腺肿大小成正比，这与T3水平或三种公认的传统甲状腺刺激抗体检测方法均无相关性。3/9例在一次或多次甲状腺切除术后复发的非毒性结节性甲状腺肿病例以及1/10例家族性单纯性甲状腺肿病例检测结果呈阳性。所有正常受试者、所有地方性甲状腺肿病例以及21例散发性非毒性结节性甲状腺肿病例检测结果均为阴性。尽管该方法不如“生长CBA”敏感，但它清楚地强调了导致格雷夫斯甲状腺毒症中甲状腺上皮细胞规律性增生的生长刺激强度与非毒性结节性甲状腺肿典型的无序且代谢不协调增生之间的本质区别。
