Hoch D H, Grodzinsky A J, Koob T J, Albert M L, Eyre D R
J Orthop Res. 1983;1(1):4-12. doi: 10.1002/jor.1100010102.
We have correlated early material and biochemical changes in articular cartilage in a surgical model for cartilage degeneration. Medial meniscectomy was performed on the left knee of 17 adult, female New Zealand white rabbits. The equilibrium Young's modulus of cartilage was assessed by an indentation test in situ at defined sites on the medial and lateral tibial plateaus of the operated and control knees; the cartilage was then excised and analyzed biochemically. Focal changes were consistently observed in the medial surface of the operated knee. The equilibrium modulus and the glycosaminoglycan content fell rapidly, reaching a minimum by 2 weeks after surgery; the lateral tibial surface was essentially unaffected. Six months after surgery, the glycosaminoglycan content had returned to normal and the modulus to near normal. Independent measurements on cored plugs from the medial surface 2 weeks after surgery revealed a significant decrease in both the dynamic stiffness and the streaming potential in the operated knee compared with the control. The findings suggest that normal ambulatory loads in vivo will deform the affected medial cartilage much more than normal. It remains to be seen if altered mechanical stresses are solely responsible for initiating and sustaining matrix remodeling by the chondrocytes.
我们在一个用于研究软骨退变的手术模型中，对关节软骨的早期物质和生化变化进行了关联研究。对17只成年雌性新西兰白兔的左膝进行内侧半月板切除术。通过压痕试验在手术侧和对照侧膝关节的内侧和外侧胫骨平台的特定部位原位评估软骨的平衡杨氏模量；然后切除软骨并进行生化分析。在手术侧膝关节的内侧表面持续观察到局部变化。平衡模量和糖胺聚糖含量迅速下降，在术后2周达到最低水平；外侧胫骨表面基本未受影响。术后6个月，糖胺聚糖含量恢复正常，模量接近正常。术后2周对手术侧膝关节内侧表面取出的芯样进行的独立测量显示，与对照相比，手术侧膝关节的动态刚度和流动电位均显著降低。这些发现表明，体内正常的行走负荷对受影响的内侧软骨的变形程度远大于正常情况。机械应力的改变是否是引发和维持软骨细胞基质重塑的唯一原因，仍有待观察。
