Yudkoff M, Nissim I, Kim S U, Pleasure D, Segal S
J Neurochem. 1984 Jan;42(1):283-6. doi: 10.1111/j.1471-4159.1984.tb09731.x.
Gas chromatography-mass spectrometry was used to study the metabolism of 15NH3 in organotypic cerebellar explants and cultured astrocyte monolayers. A steady-state level of 15NH3 was present by 1 min in both systems. Steady-state labeling in L-[amide-15N] glutamine, L-[15N]alanine, L-[15N]glutamate, and L-[15N]aspartate was attained by 1 min after 15NH3 addition in the organotypic cerebellar explants and by approximately 5 min in the cultured astrocytes. No measurable 15N labeling was noted in either glycine or serine in either system.
采用气相色谱-质谱联用技术研究了15NH3在小脑器官型外植体和培养的星形胶质细胞单层中的代谢情况。在两个系统中，1分钟时均出现了15NH3的稳态水平。在小脑器官型外植体中添加15NH3后1分钟，以及在培养的星形胶质细胞中约5分钟后，L-[酰胺-15N]谷氨酰胺、L-[15N]丙氨酸、L-[15N]谷氨酸和L-[15N]天冬氨酸达到稳态标记。在任何一个系统中，甘氨酸或丝氨酸均未检测到可测量的15N标记。
